Below follow examples of scientific papers where the M30 monoclonal antibody has been used to study apoptosis:

On the M30-Apoptosense® assay

Leers, M.P., Kölgen, W., Björklund, V., Bergman, T., Tribbick, G., Persson, B., Björklund, P., Ramaekers, F.C.S., Björklund, B., Nap, M., Jörnvall, H., and Schutte, B. (1999). Immunocytochemical detection and mapping of a Cytokeratin 18 neo-epitope exposed during early apoptosis. J Pathol 187, 567-572.

2003
Thurnher, D., Turhani, D., Pelzmann, M., Wannemacher, B., Knerer, B., Formanek, M., Wacheck, V. and Selzer, E. (2003). Betulinic Acid: A new cytotoxic compound against malignant head & neck cancer cells. Head & Neck, (in press).

Ueno, T., Toi, M., Bivén, K., Bando, H., Ogawa, T. and Linder, Stig. (2003). Measurement of an apoptosis product in the sera of breast cancer patients. European Journal of Cancer; 39 (2003): 769-774.

Bivén, K., Erdal, H., Hägg, M., Ueno, T., Zhou, R., Lynch, M., Rowley, B., Wood, J., Zhang, C., Toi, M., Shoshan, M.C. and Linder, S. (2003). A novel assay for discovery and characterization of pro-apoptotic drugs and for monitoring apoptosis in patient sera. Apoptosis 2003; 8: 263-268.

2001-2002
Linder, S., Bivén, K., Björklund, P. (2000). The M30 antibody can be used to quantitate apoptosis in breast cancer cell extracts and culture supernatants. Tumor Biology 21 (suppl 1) p. 30.

Kramer, G., Steiner, G., Memaran-Dadgar, N., Sokol, P. and Marberger, M. (2001). Determination of apoptotic activity in the sera of patients with hormone refractory prostate cancer by a novel M30 apoptosis assay. Awarded Best Poster Presentation in the session "Metastatic prostate cancer". Eur Urol 2001; 39 (suppl 5) p. 80.

Mandic, A., Viktorsson, K., Molin, M., Akusjärvi, G., Eguchi, H., Hayashi, S.-I., Toi, M., Hansson, J., Linder, S. and Shosan, M. (2001). Cisplatin induces the pro-apoptotic conformation of Bak in a delta-MEKK1-dependent manner. Mol Cell Biol 21, 3684-3691.

Ueno, T., Toi, M., Bivén, K., Björklund, P. and Linder, S. (2001). Measurement of apoptosis in serum from breast cancer patients. XXIX ISOBM Meeting, Abstract No O-53, p.120.

Hägg, M., Bivén, K., Ueno, T., Rydlander, L, Björklund, P., Wiman, K.G., Shoshan, M.C. and Linder, S. (2002) A novel high-through-put assay for screening of pro-apoptotic drugs. Invest. New Drugs, 20: 253-259.

On the M30 antibody

Leers, M.P., Kölgen, W., Björklund, V., Bergman, T., Tribbick, G., Persson, B., Björklund, P., Ramaekers, F.C.S., Björklund, B., Nap, M., Jörnvall, H., and Schutte, B. (1999). Immunocytochemical detection and mapping of a Cytokeratin 18 neo-epitope exposed during early apoptosis. J Pathol 187, 567-572.

2003
Huppertz, B., Kingdom, J.,Cannigia, I., Desoye, G., Black, S., Korr, H. and Kaufmannn, P. (2003). Hypoxia favours necrotic versus apoptotic shedding of placental syncytiotrophoblast into the material circulation Placenta (2003); 24: 181-190.

Gauthier, Y., Cario-André, M., Lepreux, S., Pain, C. And Taïeb, A. (2003). Melanocyte detachment after skin friction in non lesional skin of patients with generalized vitiligo. Br J Dermatology (2003); 148: 95-101.

Lukkarinen, H.P., Laine, J. and Kaapa, P.O. (2003). Lung epithelial cells undergo apoptosis in neonatal respiratory distress syndrome. Pediatr Res (2003) ; 53: 254-259.

Leers, M.P.G. (2003). Overzicht: Cytokeratine en zijn afbraakprodukten als potentiële aangrijpingspunten om apoptose te monitoren in patiëntserum. Ned Tijdschr Klin Chem 2003; 28: 19-24.

Thurnher, D., Turhani, D., Pelzmann, M., Wannemacher, B., Knerer, B., Formanek, M., Wacheck, V. and Selzer, E. (2003). : A new cytotoxic compound against malignant head & neck cancer cells. Head & Neck, (in press).

Ueno, T., Toi, M., Bivén, K., Bando, H., Ogawa, T. and Linder, Stig. (2003). Measurement of an apoptotic product in the sera of breast cancer patients. European Journal of Cancer; 39 (2003): 769-774.

2002
Mirzaie-Joniani, H., Eriksson, D., Sheikholvaezin, A., Johansson, A., Löfroth, P-O., Johansson, l. and Stigbrand, T. (2002). Apoptosis Induced by Low-Dose and Low-Dose-Rate Radiation. Cancer 2002; 94: 4, Supplement; 1210-1214 (Feb 15).

Backus, H.H., van Groeningen, C.J., Vos, W., Dukers, D.F., Bloemena, E., Wouters, D., Pinedo, H.M. and Peters, G.J. (2002). Differential expression of cell cycle and apoptosis related proteins in colorectal mucosa, primary colon tumours, and liver metastases. J Clin. Pathol. 2002; 55(3): 206 211.

Auten, R.L., Whorton, M.H. and Nicholas Mason, S. (2002). Blocking neutrophil influx reduces DNA damage in hyperoxia-exposed newborn rat lung. Am. J. Respir Cell Mol Biol. (2002); 26(4): 391-397.

Sugita, J., Ohtani, H., Mizoi, T., Saito, K., Shiiba, K., Sasaki, I., Matsuno, S., Yagita, H., Miyazawa, M. and Nagura, H. (2002). Close association between Fas ligand (FasL; CD95L)-positive tumor-associated macrophages and apoptotic cancer cells along invasive margin of colorectal carcinoma: a proposal on tumor-host interactions. Jpn J Cancer Res (2002); 93(3): 320-328.

Mourits, M.J., Hollema, H., de Vries, E.G., Ten Hoor, K.A., Willemse, P.H. and van der Zee, A.G. (2002). Apoptosis and apoptosis-associated parameters in relation to tamoxifen exposure in postmenopausal endometrium. Hum Pathol (2002); 33(3): 341-346.

Krzyzowska, M., Schollenberger, A., Skierski, J. And Niemialtowski, M. (2002). Apoptosis during ectromelia orthopoxvirus infection is DEVDase dependent: in vitro and in vivo studies. Microbes Infect (2002); 4(6): 599-611.

Carr, N.J., Emory, T.S. and Sobin, L.H. (2002). Epithelial neoplasms of the appendix and colorectum: an analysis of cell proliferation, apoptosis, and expression of p53, CD44, and bcl-2. Arch Pathol Lab Med (2002); 126(7): 837-41.

Shiojiri, T., Wada, K., Nakajima, A., Katayama, K., Shibuya, A., Kudo, C., Kadowaki, T., Mayumi, T., Yura, Y. And Kamisaki, Y. (2002). PPARgamma ligands inhibit nitrotyrosine formation and inflammatory mediator expressions in adjuvant-induced rheumatoid arthritis mice. Eur J Pharmacol (2002); 448(2-3): 231-238.

Sheard, M.A., Vojtesek, B. and Valik, D. (2002). Release of Cytokeratin-18 and -19 Fragments (TPS and Cyfra 21-1) Into the Extracellular Space During Apoptosis. J Cellular Biochem (2002) 85: 670-677.

Jordan, J. and Butchko, A. (2002). Apoptotic Activity in Villous Trophoblast Cells During B19 Infection Correlates with Clinical Outcome: Assessment by the Caspase-related M30 CytoDeath Antibody. Placenta (2002): 23(7): 547.

Levy, R., Smith, S.D., Yusuf, K., Huettner, P.C., Kraus, F.T., Sadovsky, Y. and Nelson, D.M. (2002). Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression. Am J Obstet Gynecol (2002); 186: 1056-1061.

Cinell, I.I., Buyukafsar, K., Cinell, L., Polat, A., Atici, S., Tamer, L. and Oral, U. (2002). The role of Poly(ADP-Ribose) synthetase inhibition in preventing endotoxemia-induced intestinal epithelial apoptosis. Pharmacol Res (2002); 46(2): 119.

Park, S.H., Kim, H.K., Kim, H. and Ro, J.Y. (2002). Apoptosis in thymic epithelial tumors. Pathol Res Pract (2002); 198(7): 461-467.

Leers, M.P., Björklund, V., Björklund, B., Jörnvall, H. and Nap, M. (2002). An immunohistochemical study of the clearance of apoptotic cellular fragments. CMLS, Cell Mol. Life Sci. 59 (2002): 1358-1365.

Lee, J.C., Schickling, O., Stegh, A.H., Oshima, R.G., Dinsdale, D., Cohen, G.M. and Peter, M.E. (2002). DEDD regulates degradation of intermediate filaments during apoptosis. J Cell Biology (2002); 158(6): 1051-1066.

Breuckmann, F., von Kobyletzki, G., Avermaete, A., Kreuter, A., Mannherz, H.G. and Altmeyer, P. (2002). Apoptosis of human dermal endothelial cells as a potential side effect following therapeutic administration of UVA1 irradiation: preliminary results. Arch Dermatol Res (2002); 294: 303-309.

Tesfaigzi, Y., Fischer, H., Dahesia, M., Green, F., De Sanctis, G. and Wilder, J. (2002). Bax is crucial for IFN-g-Induced Resolution of Allergen-Induced Mucus Cell Metaplasia. The Journal of Immunology (2002); 169: 5919-5925.

Austgulen, R., Chedwick, L., Vogt Isaksen, C., Vatten, L. and Craven, C. (2002). Trophoblast Apoptosis in Human Placenta at Term as Detected by Expression of a Cytokeratin 18 Degradation Product of Caspase. Arch Pathol Lab Med (2002); Vol 126: 1480-1486.

Wesierska-Gadek, J.,Schloffer, D., Kotala, V. and Horky, M. (2002). Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy. Int.J. Cancer (2002): 101: 128-136.

2001
Yu, D-C., Chen, Y., Dilley, J., Embry, M., Zhang, H., Nguyen, N., Amin, P., Oh, J. and Henderson, D. (2001). Antitumor Synergy of CV787, a Prostate Cancer-specific Adenovirus, and Paclitaxel and Docetaxel. Cancer Research 61: 517-525, January 15, 2001.

Fukuda, M., Tanaka, A., Kitada, M., Fukuda, F., Suzuki, S., Jiang, Y., Kebusa, Y., Ohno, J., Yamamoto, Y., Minato, H., Nonomura, A., Sakashita, H. and Kusama, K. (2001) Immunohistochemical detection of cytokeratin 18 and its neo-epitope in Warthin's tumor (adenolymphoma) of the parotid glands. Anticancer Res 2001 Jan-Feb;21(1A):109-12.

Kadyrov, M., Kaufmann, P. and Huppertz, B. (2001). Expression of a Cytokeratin 18 Neo-epitope is a Specific Marker for Trophoblast Apoptosis in Human Placenta. Placenta 2001, 22: 44-48.

Piazza, G.A., Thompson, W.J., Pamukcu, R., Alila, H.W., Whitehead, C.M., Liu, L., Fetter, J.R., Gresh, W.E. Jr, Klein-Szanto, A.J., Farnell, D.R., Eto, I. and Grubbs, C.J. (2001) Exisulind, a novel proapoptotic drug, inhibits rat urinary bladder tumorigenesis. Cancer Res 2001 May 15;61(10):3961-8.

Backus, H.H., van Riel, J.M., van Groeningen, C.J., Vos, W., Dukers, D.F., Bloemena, E., Wouters, D., Pinedo, H.M., and Peters, G.J. (2001). Rb, mcl-1 and p53 expression correlate with clinical outcome in patients with liver metastases from colorectal cancer. Ann Oncol 2001 Jun;12(6):779-85.

Michael-Robinson, J.M., Biemer-Hüttmann, A-E., Purdie, D.M., Walsh, M.D., Simms, L.A., Biden, K.G., Young, J.P., Leggett, B.A., Jass, J.R. and Radford-Smith, G.L. (2001). Tumour infiltrating lymphocytes and apoptosis are independent features in colorectal cancer stratified according to microsatellite instability status. Gut 2001; 48: 360-366.

Bantel, H., Ruck, P., Gregor, M. and Schulze-Osthoff, K. (2001). Detection of elevated caspase activation and early apoptosis in liver diseases. European Journal of Cell Biology 80, 230-239, March 2001.

Chiu, P.M., Ngan, Y.S., Khoo, U.S. and Cheung, A.N. (2001). Apoptotic activity in gestational trophoblastic disease correlates with clinical outcome: assessment by the caspase-related M30 CytoDeath antibody. Histopathology 2001 Mar;38(3):243-9.

Roy, H. K., Karoski, W. J., Ratashak, A. and Smyrk, T. C. (2001). Chemoprevention of intestinal tumorigenesis by nabumetone: induction of apoptosis and Bcl-2 downregulation. British Journal of Cancer Vol. 84, No. 10; 1412-1416, May 1, 2001.

Backus, H.H., Dukers, D.F., van Groeningen, C.J., Vos, W., Bloemena, E., Wouters, D., van Riel, J.M., Smid, K., Giaccone, G., Pinedo, H.M. and Peters, G.J. (2001). 5-Fluorouracil induced Fas upregulation associated with apoptosis in liver metastases of colorectal cancer patients. Ann Oncol 2001 Feb;12(2):209-16.

Backus, H.H., van Riel, J.M., van Groeningen, C.J., Vos W., Dukers, D.F., Bloemena, E., Wouters, D., Pinedo, H.M. and Peters, G.J. (2001). Rb, mcl-1 and p53 expression correlate with clinical outcome in patients with liver metastases from colorectal cancer. Ann Oncol 2001 Jun; 12(6): 779-785.

Coffey, J.C., Bennett, M.W., Wang, J.H., O'Connell, J., Neary, P., Shanahan, F., Redmond, H.P. and Kirwan, W.O. (2001). Upregulation of Fas-Fas-L (CD95/CD95L)-mediated epithelial apoptosis--a putative role in pouchitis? J Surg Res 2001 Jun 1;98(1):27-32.

Barrett, K.L., Willingham, J.M., Garvin, A.J. and Willingham, M.C. (2001). Advances in cytochemical methods for detection of apoptosis. J Histochem Cytochem 2001 Jul;49(7):821-32.

Riesenberg, R., Buchner, A., Pohla, H. and Lindhofer, H. (2001). Lysis of prostate carcinoma cells by trifunctional bispecific antibodies (alpha EpCAM x alpha CD3). J Histochem Cytochem 2001 Jul;49(7):911-7.

Song, E., Su, F, Chen, J., Ou, Q., Wang, M. and Exton, M.S. (2001). Blocking CTL-based cytotoxic pathways reduces apoptosis of transplanted hepatocytes. J Surg Res 2001 Jul;99(1):61-9.

Ferrandina, G., Ranelletti, F.O., Larocca, L.M., Maggiano, N., Fruscella, E., Legge, F., Santeusanio, G., Bombonati, A., Mancuso, S. and Scambia, G. (2001). Tamoxifen modulates the expression of Ki67, apoptosis, and microvessel density in cervical cancer. Clin Cancer Res 2001 Sep;7(9):2656-61.

Ferrandina, G., Filippini, P., Ferlini, C., Maggiano, N., Stoler, A.B., Fruscella, E, Mozzetti, S., Mancuso, S. Freeedman, R.S., Scambia, G. and Ranelletti, O. (2001). Growth inhibitory effects and radiosensitization induced by fatty aromatic acids on human cervical cancer cells. Oncology Res 2001 ; 12: 429-440.

Walker, J.A. and Quirke, P. (2001). Viewing apoptosis through a "TUNEL". J.Pathol 195: 275-276 (2001).

Thurnher, D., Bakroeva, M., Schütz, G., Pelzman, M., Formanek, M., Knerer, B., and Kornfehl, J. (2001). Non-steroidal anti-inflammatory drugs induce apoptosis in head and neck cancer cell lines. Acta Otolaryngol 2001; 121; 957-962.

2000
Farina, F., Cappello, F., Todaro, M., Bucchieri, F., Peri, G., Zummo, G. and Stassi, G. (2000). Involvement of caspase-3 and GD3 ganglioside in ceramide-induced apoptosis in Farber disease. J Histochem Cytochem 2000 Jan;48(1):57-62.

Kusama, K., Jiang, Y., Toguchi, M., Ohno, J., Shikata, H., Sakashita, H. and Sakagami, H. (2000). Use of the monoclonal antibody M30 for detecting HSG cell apoptosis. Anticancer Res 20, 151-154.

Kusama, K., Jiang, J., Shikata, H., Ishikawa, F., Taguchi, K., Kikuchi, K., Mori, K., Sakashita, H., Sakagami, H., Kaneko, T.and Yamamoto, Y. (2000). Immunohistochemical detection of cytokeratin 18 and its neo-epitope in human salivary glands and pleomorphic adenomas. Anticancer Res 20 (4), 2485-2487.

MacFarlane, M., Merrison, W., Dinsdale, D. and Cohen, G.M. (2000). Active caspases and cleaved cytokeratins are sequestered into cytoplasmic inclusions in TRAIL-induced apoptosis. J Cell Biol 148, 1239-1254.

Morsi, H.M., Leers, M.P., Jäger, W., Björklund, V., Radespiel-Troger, M., el Kabarity, H., Nap, M. and Lang, N. (2000). The patterns of expression of an apoptosis-related CK18 neoepitope, the bcl-2 proto-oncogene, and the Ki67 proliferation marker in normal, hyperplastic, and malignant endometrium. Int J Gynecol Pathol 19, 118-126.

Morsi, H.M., Leers, M.P., Radespiel-Troger, M., Björklund, V., Kabarity, H.E., Nap, M.and Jäger, W. (2000). Apoptosis, bcl-2 expression, and proliferation in benign and malignant endometrial epithelium: An approach using multiparameter flow cytometry. Gynecol Oncol 2000 Apr; 77(1):11-7.

Sakagami, H., Jiang, Y., Kusama, K., Atsumi, T., Ueha, T., Toguchi, M., Iwakura, I., Satoh, K., Ito, H., Hatano, T., and Yoshida, T. (2000). Cytotoxic activity of hydrolyzable tanninns against human oral tumor cell lines --- A possible mechanism. Phytomed 7, 39-47, 2000.

Sakagami, H., Jiang, Y., Kusama, K., Atsumi, T., Ueha, T., Toguchi, M., Iwakura, I., Satoh, K., Fukai, T. and Nomura, T. (2000). Induction of apoptosis by flavones, flavonols (3-hydroxyflavones) and isoprenoid-substituted flavonoids in human oral tumor cell lines. Anticancer Res 20, 271-277.

Terasaka, H., Tamura, A., Takayama, F., Kashimata, M., Ohtomo, K., Macino, M., Fujisawa, S., Toguchi, M., Kanda, Y., Kunii, S., Kusama, K., Ishino, A., Watanabe, S., Takano, H., Takahama, M. and Sakagami, H. (2000). Induction of apoptosis by dopamine in human oral tumor cell lines. Anticancer Res 20, 243-250.

Morsi, H.M., Leers, M.P., Nap. M., Björklund, V., El Kabarity, H. and Jäger W. (2000). Apoptosis and anti-apoptosis in oestrogen-receptor negative endometrial cancer cells in response to anastrozole, 4-hydroxitamoxifen and medroxyprogesterone acetate. Eur J Cancer 36 spuppl: 112-113.

Hotchkiss, R.S., Schmieg, R.E. Jr., Swanson, P.E., Freeman, B.D., Tinsley, K.W., Cobb, J.P., Karl, I.E. and Buchman, T.G. (2000). Rapid onset of intestinal epithelial and lymphocyte apoptotic cell death in patients with trauma and shock. Crit Care Med 2000 Sep;28(9):3207-17.

Kronenberger, B., Rüster, B., Lee, J-H., Sarrazin, C., Roth, W.K., Herrmann, G. and Zeuzem, S. (2000). Hepatocellular proliferation in patients with chronic hepatitis C and persistently normal or abnormal aminotransferase levels. J Hepatology 2000; 33: 640-647.

Vermes, I., Haanen, C. and Reutelingsperger, C. (2001) Flow cytometry of apoptotic cell death. J Immunol Methods 2000 Sep 21;243(1-2):167-90.

Carr, N.J. (2000). M30 Expression Demonstrates Apoptotic Cells, Correlates With In Situ End-Labeling, and Is Associated With Ki-67 Expression in Large Intestinal Neoplasms. Arch Pathol Lab Med; 124: 1768-1772, December 2000.

Weusten, A., Groothuis, P., Dunselman, G., Goeij, A., Arends, J. and Evers, J. (2000). Morphological changes in mesothelial cells induced by shed menstrual endometrium in vitro are not primarily due to apoptosis or necrosis. Human Reproduction; 15 (7): 1462-1468.

Jiang, Y., Kusama, K., Satoh, K., Takayama, E., Watanabe, S. and Sakagami, H. (2000). Induction of cytotoxicity by chlorogenic acid in human oral tumor cell lines. Phytomedicine Dec;7(6):483-91.

Bantel, H., Ruck, P. and Schulze-Osthoff, K. (2000). In situ monitoring of caspase activation in hepatobiliary diseases. Letter to the Editor. Cell Death and Differentiation (2000) 7, 504-505.

1999
Stabler, S.M., Ostrowski, L.L., Janicki, S.M., and Monteiro, M.J. (1999). A myristoylated calcium-binding protein that preferentially interacts with the Alzheimer´s disease presenilin 2 protein. J Cell Biol 145, 1277-1292.

Ishino, A., Kusama, K., Watanabe, S., and Sakagami, H. (1999). Inhibition of epigallocatechin gallate-induced apoptosis by CoCl2 in human oral tumor cell lines. Anticancer Res 19, 5197-5202.

Kawase, M., Sakagami, H., Kusama. K., Motohashi, N. and Satoh, K. (1999). a-trifluoromethylated acyloins induce apoptosis and caspase 3-like protease activity. Bioorg Med Chem Lett 9 (21), 3113-3118.

Sakagami, H.,Kusama, K., Toguchi, M., and Kochi, M. (1999). Induction of non-apoptotic cell death by sodium 5,6-benzylidine-L-ascorbate in a human salivary gland tumor cell line. Anticancer Res 19, 4045-4048.

Ebert, A.D., Wechselberger, C., Frank, S., Wallace-Jones, B., Seno, M., Martinez-Lacaci, I., Bianco, C., De Santis, M., Weitzel, H.K. and Salomon, D.S. (1999). Cripto-1 induces phosphatidylinositol 3'-kinase-dependent phosphorylation of AKT and glycogen synthase kinase 3beta in human cervical carcinoma cells. Cancer Res 1999 Sep 15;59(18):4502-5.